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KMID : 0379520220380040523
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한국독성학회지
2022 Volume.38 No. 4 p.523 ~ p.530
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Sublethal pulmonary toxicity screening of silica nanoparticles in rats after direct intratracheal instillation
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Han Hyoung-Yun
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Abstract
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The present aimed to characterize the toxicity of silica nanoparticles in Sprague Dawley rats and determine the dose levels for a repeated-dose toxicity study. Silica nanoparticles (SiO2, 20 nm and 50 nm) were administered as a single intratracheal instillation of standardized SiO2 20 nm (low dose, 200 μg/mL; high dose, 400 μg/mL) and 50 nm (low dose, 200 μg/mL; high dose, 400 μg/mL). Each group consisted of five male rats. We documented the mortality rate, clinical signs, body weight, bronchoalveolar lavage fluid analysis, hematological values, serum chemistry values, organ weight, gross findings at necropsy, and histopathological assessments. Rats treated with 200 μg/mL and 400 μg/mL SiO2 50 nm exhibited a decreased mean corpuscular volume, while those treated with 400 μg/mL of SiO2 50 nm showed increases in absolute monocyte and absolute lymphocyte count as well as prothrombin time. In addition, rats treated with 400 μg/mL SiO2 20 nm and 50 nm presented reduced creatinine, alanine aminotransferase, and sodium levels. Therefore, a single intratracheal instillation of SiO2 20 nm and 50 nm elicited no toxicity up to a dose of 400 μg/mL, and the approximate lethal dose of this test substance exceeded 400 μg/mL in male Sprague Dawley rats under the present experimental conditions.
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KEYWORD
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Silica nanoparticles, Intratracheal instillation, SD rat
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